IMPLDEBRIS-PI-3RL-IINFACTS-2019: Biological assessment of debris from oral implants.

Principal Investigator: 
Hassan Bousbaa (
Leader Institution: 
Research Team: 
Hassan Bousbaa (PI), Primavera Santos (Co-PI), Bruno Sarmento (Co-PI), Flávia Sousa, Rui Moura, Andreia Almeida, Antonio Pedro Fonseca, Júlio César Matias de Souza, Ana Henriques, Patrícia Silva, Joel Pedrosa, Henrique Borges, Ana Borges
Funding entity: 
Period covered: 
03/2019 - 02/2020

Dental implants and orthodontic mini-implants manufactured from titanium and stainless-steel alloys are routinely used in clinical practice. Most orthodontic mini-implants are manufactured from Titanium alloy (Ti6Al4V) or stainless-steel alloys although several constituent elements of these alloys reveal a mutagenic or carcinogenic potential. The aim of the present study is to carry out an in vitro cytotoxic analysis in order to assess the biological safety of particles released from titanium alloys and stainless-steel orthodontic mini-implants. During the first year of the project, these particles will be characterized in terms of size and morphology, and their cytotoxic effect analyzed in terms of cell viability and permeability. During the second year, the particles will be characterized in terms of cellular internalization mechanism and genotoxicity potential. It is expected that this study will contribute to clarify whether titanium wear-derived particles released in the oral environment are associated with harmful side effects in the human body.


Project area: