Fingerprint analysis constitutes one of the main tools used in forensic evidence, allowing to identify the author of a certain fingerprint, tying him into a crime scene/object. Fingerprints result mainly from the deposition of human secretions on a surface, some of their constituents being already characterized. However, the composition of latent fingerprints varies over time due to chemical, biological and physical processes. Despite the studies on the variation over time of few constituents, a specific metabolic marker which time-dependent variation can provide further clues on the age of a fingerprint remains to be identified. The current project will primarily allow the identification of specific chemical markers, aiming the development of a protocol for the determination of the age of a latent fingerprint. Additionally, the interindividual variation (gender and race) of selected metabolic markers will be determined, potentiating its use in a discriminative analysis. Consequently, the knowledge on the chemical composition of aged fingerprints will be deepened, simultaneously paving the way for the development of new chemical methods for their age dating.