In recent years, some gut bacteria like Faecalibacterium prausnitzii and Akkermansia muciniphila, been identified from microbiome studies as potential probiotics or therapeutic microorganisms that could be used to treat or prevent inflammatory and diet-related physiological disorders. However, these microorganisms are difficult to handle because of their sensitivity of oxygen traces and low pH and so their successful use in food dietary supplements or drugs will depend on their stability and efficacy in humans. This project proposes to use microencapsulation to improve the viability and stability at atmospheric air and to achieve the intestinal delivery of these probiotics. Microfluidic-based techniques will be developed to encapsulate the model bacteria Bifidobacterium longum (a gut bacterium sensitive to oxygen and low pH values) in a polysaccharide-based matrix supplemented with protective agents (antioxidants and prebiotics). Different types of microfluidic devices and formulations will be studied. The microcapsules developed will be characterized and tested for stability, viability and control delivery of the beneficial microorganism.