22 de outubro, 2020 | 12h00 | Webinar – Plataforma ZOOM
Resumo da apresentação:
The use of fluoroquinolone antibiotics for therapeutic, prophylactic and growth promotion applications is increasing worldwide. Due to its halogenated characteristics, ecotoxicity and persistence, a special importance is given to their occurrence in the environment. Since some fluoroquinolones have one or more stereogenic centers, they give rise to pairs of enantiomers. The pharmacological properties and toxicity of enantiomers can be very different. The stereochemistry of these compounds represents a major challenge in terms of its environmental monitoring and is frequently ignored. The presence of fluoroquinolones has been described in many environmental matrices, but few studies were done in Portugal regarding environmental monitoring studies.
Degradative processes for the removal of fluoroquinolones have also been subject of several studies, but none has considered the relevance of the stereochemistry. This work comprises the development of new analytical methods using liquid chromatography with fluorescence detection (LC-FD) to assess biodegradation of chiral and achiral fluoroquinolone antibiotics and liquid chromatography tandem mass spectrometry (LCMS/MS) to quantify fluoroquinolones residues in different environmental matrices, such as influents and effluents of waste water treatment plants. As the metabolites/degradation products originated during degradation processes may present higher ecotoxicity than the parent compound this work also aimed to identify the most important by-products originated during these processes. Fluoroquinolones by-products formed during degradation assays were identified using an integrated analytical approach and these studies enabled the proposal of the chemical structures of two new metabolites for ofloxacin and norfloxacin, never reported before. Biodegradation of chiral fluoroquinolone antibiotics in laboratory experiments using activated sludge and two single bacterial strains were followed by the enantioselective methods developed. An analytical method for quantification and achiral assessment of fluoroquinolones in environmental matrices was developed and applied to wastewater treatment plants influents and effluents.
Biography:
Alexandra S. Maia graduated in Environmental Health and Biotoxicology in 2009 at Instituto Universitário de Ciências da Saúde (IUCS). In 2011, she obtained the Master degree in Environmental Contamination and Toxicology at ICBAS - University of Porto, and she was selected for a research grant under the “FLUOROPHARMA” project, at the associate laboratory CBQF (Centre of Biotechnology and Fine Chemistry) from Catholic University of Portugal (UCP). In 2018, she concluded her PhD degree in Biotechnology (specialization in Environmental Science and Engineering), in the area of Environmental Analytical Chemistry. She has published 21 peer-reviewed journal papers, five of which as the first author, and has 29 conference proceedings. In February of 2019, she was selected as a junior researcher at the Laboratory of Catalysis and Materials (LCM), Faculdade de Engenharia da Universidade do Porto, under the project SAMPREP, under the supervision of Dr. Ana Rita Ribeiro. Since March 2020 she is a full-time assistant professor at IUCS (Instituto Superior de Ciências da Saúde). Current Research Interests: Development of LC-MS/MS methods for the determination of organic micropollutants; Occurrence studies of multi-class compounds in the environment; Development of novel eco-friendly sample preparation methodologies; Identification of by-products in environmental matrices.
O Seminário em referência é gratuito.
Para assistir basta aceder ao link abaixo, no dia e hora agendada.
Link de acesso: https://zoom.us/j/95966397845
Webinar ID: 95966397845
IINFACTS - Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde CBAS_4Ed. - Programa Doutoral em Ciências Biológicas Aplicadas à Saúde do IUCS
23 de outubro, 2020 | 12h00 | Webinar – Plataforma ZOOM
Resumo da apresentação:
Human gait analysis has been studied to recognise disorders caused by neurological, metabolic and traumatic diseases, as well as to assess the efficacy of rehabilitation treatments. The study of human gait has particular importance to provide useful information to the clinical staff, to stablish the rehabilitation processes and to provide faster and more specific treatments.
Gait indices have increasingly been used to objectively analyse the gait quality in order to understand the severity of these disorders, to distinguish between normal and abnormal gait patterns and also to quantify the deviations from the normal pattern in terms of the number of standard deviations. The graphical analysis of these indices allows to identify the less recovered limb and the correspondent stride durations, assisting doctors to better adapt the rehabilitation procedures for each patient.
This methodology can also be used to assess the arthrosis risk, which is a common gait disorder.
The possibility of using objective strategies to assess the quality of the rehabilitation process and to develop strategies to prevent early knee osteoarthritis is an important issue to human life quality.
Biography:
Manuel M. Crisóstomo is a Researcher at ISR - Institute of Systems and Robotics - and a lecturer at Department of Electrical Engineering - University of Coimbra, teaching Quality Control and Maintenance, Industrial Automation and Robotics.
Crisóstomo has received the B.Sc. degree from the University of Coimbra in 1978, the M.Sc. degree from the Technical University of Lisbon in 1987 and, in 1992, the Ph.D. degree from Brunel University, Uxbridge, U.K.
O Seminário em referência é gratuito.
Link de acesso: https://zoom.us/j/98768157496
Webinar ID: 98768157496
IINFACTS - Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde CBAS_4Ed. - Programa Doutoral em Ciências Biológicas Aplicadas à Saúde do IUCS
15 de outubro, 2020 | 12h00 | Webinar – Plataforma ZOOM
Resumo da apresentação:
Community oral health is an essential research field for dental practice. It allows the integration of population dental needs, research in population dental education and allows the intervention of dental practitioners in population dental behaviors.
Through the study of public oral health, we can achieve and integrate different measures and means that can be used to prevent both dental and general health problems.
This knowledge allows the definition of strategies for oral education and oral health literacy among the populational oral health.
This presentation aims to advertise the research that has been carried out within the Faculty of Dental Medicine of the Universidade Católica Portuguesa research group which were obtained through field observations and data integration. Effects and associations of oral and gastric cancer Helicobacter pylori, and also community oral behaviors and its consequences in public oral health education are examples of the developed studies.
Helicobacter pylori (H. pylori)—a gram-negative, microaerophilic, rod-shaped bacterium that colonizes the human stomach. It is an important bacterium species that may have an important pathobiological role, especially in the gastric mucosa.
It resides beneath the gastric mucous layer, adjacent to the gastric epithelial cells and, although it is not invasive it can give rise to chronic inflammation of the gastric mucosa. Infection with this organism is now recognized as a serious, transmissible infectious disease highly associated with the development of chronic and atrophic gastritis, duodenal ulcers, gastric mucosa-associated lymphoid tissue (MALT) lymphoma and gastric carcinoma.
In order to verify the existence of an oral reservoir for H. pylori, and his role in gastric cancer, a study with Portuguese adolescents was carried out. We also aim to publish some of the educational and literacy of oral health strategies that were accomplished along the developed researches.
Biography:
Nélio Jorge Veiga is an investigator at the Universidade Católica Portuguesa. After obtaining his degree in Dental Medicine at the Universidade Católica Portuguesa in 2006, he obtained his master´s degree in public health at Universidade of Porto in 2010. He completed his PhD in Biomedicine at Universidade da Beira Interior (UBI) and since then he became assistant professor at master degree in Dental Medicine and Biomedical Science graduation at Universidade Católica. Nowadays, he is clinical director of the University Dental Clinic of the Universidade Católica Portuguesa and coordinator of the master degree in Dental Medicine at the Universidade Católica Portuguesa, since 2018. As the result of his important researches in oral public health and education, proved by 100 scientific presentations and conferences in national and international congresses, and more than 50 published scientific papers, he is also the responsible teacher of community oral health of the master degree in dental medicine of the Faculty of Dental Medicine of the Universidade Católica Portuguesa.
O Seminário em referência é gratuito.
Para assistir basta aceder ao link abaixo, no dia e hora agendada.
Link de acesso: https://zoom.us/j/93463828067
Webinar ID: 93463828067
IINFACTS - Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde CBAS_4Ed. - Programa Doutoral em Ciências Biológicas Aplicadas à Saúde do IUCS
13 de outubro, 2020 | 08:30 | Formato on-line via Plataforma Zoom
Prof. Júlio Souza
Prof. Hassan Bousbaa
Os interessados em assistir ao evento devem aceder ao seguinte link:
https://videoconf-colibri.zoom.us/j/82679321004
09 de outubro, 2020 | 12h00 | Webinar – Plataforma ZOOM
Resumo da apresentação:
How membranous organelles are formed and regulated is one the fundamental questions of eukaryotic cell biology. Scientists have addressed these questions by characterizing the structural elements of cellular organelles and introducing dynamic ques that facilitate analysis of organelle interactions. This can be achieved through the stimulation of eukaryotic cells with various reporter-cargo molecules, pharmacological inhibitions and genetic manipulations that facilitate selective perturbations and labelling of cellular structures. In this context, the biology of intracellular pathogens early emerged as an important field to gain insight into eukaryotic cell functioning. In particular, the interactions between host cells and intracellular bacteria, secreted toxins and/or enveloped virus, involve precise and highly regulated processes which can be exploited as tools to better understand cell biology. The study of host-pathogen interactions has therefore contributed greatly to our understanding of many disease-relevant mammalian cell process, including secretion, vesicular transport, autophagy, membrane repair and cellular immunity. Here we will discuss how cellular microbiology impacted our understanding of human cell biology and analyze particular examples where the study of intracellular bacteria (e.g Salmonella or Listeria spp) and enveloped virus (e.g Coronavirus) yielded ground-breaking contributions.
Biography:
Senior Scientist at Van der Goot Lab, Global Health Institute, Faculté des Sciences de la Vie, EPFL – Lausanne, Switzerland.
2013 – 2018 - Post-doctoral fellow (EMBO & FCT) at the Group of Molecular Microbiology-IBMC-I3S, University of Porto.
2006 - 2011 - PhD in Molecular Microbiology - Imperial College, CMBI, London UK
Thesis – “Interactions between Salmonella and ubiquitin pathways”.
2003 - 2006 – MSc -Medical Microbiology University Nova of Lisbon, Portugal
Thesis – “mRNA degradation studies: implications with nucleotide salvation, transcription and proteolysis”.
1999 – 2003 - Licence in Microbiology - Catholic Portuguese University – Porto, Portugal Superior School of Biotechnology
Thesis – ‘’Characterization of HPV virus in blood cells of patients with cervical cancer’’
https://www.epfl.ch/labs/vdg/van-der-goot-lab-laboratory-of-cell-and-membrane-biology/research/
O Seminário em referência é gratuito.
Para assistir basta aceder ao link abaixo, no dia e hora agendada.
Link de acesso: https://zoom.us/j/96938391801
Webinar ID: 969 3839 1801
IINFACTS - Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde CBAS_4Ed. - Programa Doutoral em Ciências Biológicas Aplicadas à Saúde do IUCS
08 de outubro, 2020 | 11h00 | Sala 3042
Docentes e Não Docentes CESPU
Este evento é gratuito mas de inscrição obrigatória.
Inscrições em: https://inscricoes.cespu.pt/
IINFACTS - Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde CBAS_4Ed. - Programa Doutoral em Ciências Biológicas Aplicadas à Saúde do IUCS
CESPU, Campus Universitário de Gandra
R. Central de Gandra, 1317
4585-116 Gandra, Paredes
02 de outubro, 2020 | 12h00 | Webinar – Plataforma ZOOM
Resumo da apresentação:
Bones are organs made from a living and dynamic tissue that suffers constant changes throughout the life course, being modeled and remodeled by growth, ageing, hormonal status, nutrition, pathology among many other factors. The overall increase in life expectancy observed in developed countries in the last decades, has helped to put into perspective the problems arising from poor skeletal health. Bone fragility is now a major health concern, as the increased risk of bone fractures has devastating outcomes in terms of mortality, decreased autonomy, and public healthcare costs. Unfortunately, and despite the recent introduction of new drugs, pharmacological treatments for improving bone health are still far from perfect both in terms of bone fracture reduction and safety profile. More than a century ago, Julius Wolf, a German surgeon, described the differences observed between bones from patients that were exposed to regular mechanical stimulation in opposition to bones that received no such regular mechanical stimulus and noted that the former were substantially more robust. A century after these first observations we now have a much more comprehensive understanding about the beneficial effects that mechanical stimulation, namely through regular exercise and physical activity, has on bone health. This knowledge can now be used to more effectively design preventive and therapeutic physical interventions to tackle skeletal health problems in several populations at risk on developing bone fractures. In this presentation, we will briefly discuss how physical activity and exercise exerts its beneficial effects on bone health and what are the main anatomical and physiological adaptations induced by it. In particular, we will discuss how regular physical activity and a sedentary behavior might influence bone loss associated with ageing, menopause, chemotherapy and bariatric surgery and also if exercise is always a straightforward good therapeutic option or not.
Biography:
Hélder Fonseca is currently an Assistant professor of Physiology and Pathology at Faculty of Sport, University of Porto. He first graduated in Sport Sciences in 2005 and received his PhD in Physical Activity and Health in 2011 and his Master’s degree in Medicine in 2016, all at the University of Porto. Since 2007 he has been interested on research on the effects that physical activity, exercise and sedentary behavior have on health and disease, with a main focus on bone quality, obesity and bariatric surgery.
O Seminário em referência é gratuito.
Para assistir basta aceder ao link abaixo, no dia e hora agendada.
Link de acesso: https://zoom.us/j/94295578778
Webinar ID: 942 9557 8778
IINFACTS - Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde CBAS_4Ed. - Programa Doutoral em Ciências Biológicas Aplicadas à Saúde do IUCS
24 de setembro, 2020 | 12h00 | Webinar – Plataforma ZOOM
Resumo da apresentação:
Colorectal cancer (CRC) is the third most common and the second deadliest type of cancer worldwide, urging the development of more comprehensive models and of more efficient treatments. Although the combination of nanotechnology with chemo- and immuno-therapy has represented a promising treatment approach, its translation to the clinic has been hampered by the absence of cellular models that can provide reliable and predictive knowledge about the in vivo efficiency of the formulation. Thus, a 3D model based on CRC multicellular tumor spheroids (MCTS) model was developed by combining epithelial colon cancer cells (HCT116), human intestinal fibroblasts and monocytes. Polymeric nanoparticles loaded with chemotherapeutics were produced and tested both in 2D cultures and in the MCTS 3D model.
Biography:
Dr. Tomás Bauleth-Ramos holds a MSc in Pharmaceutical Sciences (2014, University of Coimbra, Portugal) and a Ph.D. in Biomedical Sciences (2020) from Instituto de Ciências Biomédicas Abel Salazar (ICBAS), University of Porto (Portugal) in collaboration with Harvard University (USA) and the University of Helsinki (Finland). As of September 2020, he is author of 10 peer-reviewed journal articles (3 as first author), 2 book chapters, 9 presentations at scientific meetings, with over 260 citations. His scientific interests are related to the development of nanosystems for combined cancer chemo- and immunotherapy and the development of 3D in vitro cell culture models to assess the biocompatibility and efficacy of the developed therapies.
O Seminário em referência é gratuito.
Para assistir basta aceder ao link abaixo, no dia e hora agendada.
Link de acesso: https://zoom.us/j/95579052430
Webinar ID: 955 7905 2430
IINFACTS - Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde CBAS_4Ed. - Programa Doutoral em Ciências Biológicas Aplicadas à Saúde do IUCS
18 de setembro, 2020 | 15h00 | Webinar – Plataforma ZOOM
Resumo da apresentação:
The human body reacts to injurious stimuli by mounting an inflammatory response. In its acute phase, transvascular extravasation of fluid, plasma proteins and leukocytes occur in the microvasculature of the injured tissue. Inappropriate leukocyte infiltration into tissues associated with stroke, multiple sclerosis, obesity, and other inflammatory disorders have been postulated to cause tissue dysfunction.
The role of vascular endothelial cells as a regulatory organ in immunity and haemostasis has become well established in the last two decades. At sites of injury and inflammation, endothelium becomes locally “activated”. Visualization of leukocyte–endothelial cell interactions in vivo, by intravital microscopy, in inflamed vessels has revealed that leukocytes must first tether and roll along the venule before they can firmly adhere and emigrate out of the vasculature.
The central nervous system is considered an immunologically privileged site due to the presence of the endothelial blood–brain barrier (BBB), which, under physiological conditions, protects against leukocyte traffic into the CNS. However, during inflammatory conditions of the CNS, such as Multiple Sclerosis, large numbers of mononuclear cells gain access to the CNS. Migration of leukocytes through the BBB represents a critical pathogenetic event in the initiation of CNS inflammation.
During the lecture, previous and recent data related to molecular and cellular process (in vivo leukocyte-endothelium interactions) associated to different neuroinflammatory contexts (sepsis and MS) will be presented. The importance of using intravital microscopy technique to understand leukocyte trafficking and other vascular events will be also highlight.
Biography:
She completed her bachelor degree in Immunology & Biochemistry/Biological Sciences/ICB/UFMG and her PhD in Molecular Biology and Physiology Program at Universidad de Valladolid (Valladolid/Spain/Supervisor Dr Mariano Sanchez Crespo). She also was postdoctoral fellow at Cleveland Clinic, Lerner Research Institute (Cleveland, Ohio, USA, Supervisor Dr Damir Janigro); Instituto de Biologia y Genética Molecular (IBGM)/Universidad de Valladolid (Spain/Supervisor Maria Luiza Nieto), and also at Immunology Group/Health Science Centre/University of Calgary (Calgary, Canada/Supervisor Dr Paul Kubes). She is currently Associate Professor IV of the Department of Physiology and Biophysics, and sub-coordinator of the Professional Master's Program in Teaching Biology (PROFBIO) / ICB / UFMG. She got national and international awards at scientific events and from 1st Americas School of Neuroimmunology (ASNI/Calgary, Canada), and also received the title of Honor Professor of XIII Class of Nutrition Undergraduate Course/ UFMG.
She supervised undergraduate, master and PhD, and coordinates and collaborates in scientific projects, mainly in the area of neuroinflammation, publishing the data in indexed international journals, besides chapters and book. She also supervises research projects about Neurosciences and education. She coordinates social AGERE Program (Group of Educational Actions Related to Epilepsy), which received a UFMG recognition as the best Program in the health area (2019). She is permanent member of graduate Program in Physiology and Pharmacology, Graduate Program in Neurosciences, and Professional Master's Program in Teaching Biology (PROFBIO / ICB / UFMG).
Gratuito
Para assistir basta aceder ao link abaixo, no dia e hora agendada.
Link de acesso: https://zoom.us/j/91672663230
Webinar ID: 916 7266 3230
IINFACTS - Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde CBAS_4Ed. - Programa Doutoral em Ciências Biológicas Aplicadas à Saúde do IUCS
17 de setembro, 2020 | 12h00 | Webinar – Plataforma ZOOM
Resumo da apresentação:
Synthetic Cannabinoids (SCs) comprise a diverse group of new psychoactive substances (NPS) that bind and activate cannabinoid receptors (e.g. CB1, CB2) with higher potency than tetrahydrocannabinol (THC, the main psychoactive element of cannabis). Despite being initially designed for therapeutic applications, SCs’ increased potency and longer‐lasting psychoactive effects compared to THC favored their recreational use. However, SCs also promote stronger adverse effects compared to THC. In fact, the recreational use of SCs has become a major challenge for public health and policy-makers due to the increasing number of SC-related acute intoxications and deaths. SC use by pregnant women and women of child-bearing age is especially alarming due to the potential onset of neurodevelopmental disorders in the offspring. Within the scope of the ongoing NeuroSCANN project, our team has assessed the neurotoxic potential of distinct SCs, particularly focusing on how they affect in vitro neurogenic processes in NG108-15 neuroblastoma x glioma cells, following exposure to SCs at non-toxic, biologically relevant concentrations (≤ 1 nM), either in acute or repeated exposure settings. We reported first-hand that two commonly reported SCs, THJ-2201 and 5F-PB22, enhanced differentiation ratios and total neurite length of NG108-15 cells near 2-fold compared to vehicle-treated cells, in a CB1R activation-dependent way, as CB1 receptor blockade with a specific antagonist abrogated SC-induced effects. Interestingly, at those same concentrations, the SCs tested increased mitochondrial membrane potential but did not affect other toxicological parameters like cell viability, proliferation or intracellular ATP levels. Nevertheless, further research is required to identify the action mechanisms involved in SC-mediated increase of neurodifferentiation.
Biography:
João Pedro Silva (JP Silva) graduated in Applied Biology (2002) at the University of Minho. He further holds an MSc in Molecular Genetics (2004) and a PhD in Biological Sciences (2009) from the same University (Biology Department), having assessed the pharmacological potential of de novo-synthesized antioxidants for oxidative stress-related pathologies. As a Post-Doctoral Researcher (2010-2016), whose work spanned the Centre of Biological Engineering (UMinho), the University Medical Centre Groningen (Netherlands) and University of California Riverside (USA), JP Silva tackled prevailing medical challenges related to wound healing and tuberculosis, having been awarded an Honor Mention in the Bluepharma/UCoimbra 2016 Innovation Award and filing an international patent. Since 2016, JP Silva is a Research Associate at the Toxicology Lab, Faculty of Pharmacy (Univ. Porto) focused on assessing the toxicity of new psychoactive substances (NPS), namely in understanding the role of synthetic cannabinoids in the onset of neurodevelopmental disorders and identify potential therapeutic targets for such pathologies.
Gratuito
Para assistir basta aceder ao link abaixo, no dia e hora agendada.
Link de acesso: https://zoom.us/j/91882661979
Webinar ID: 918 8266 1979
IINFACTS - Instituto de Investigação e Formação Avançada em Ciências e Tecnologias da Saúde CBAS_4Ed. - Programa Doutoral em Ciências Biológicas Aplicadas à Saúde do IUCS